UV-1900

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UV-1900

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    The UV-1900 is a double-beam UV-Vis spectrophotometer using Shimadzu's original LO-RAY-LIGH™ diffraction grating technology. In addition to its high optical performance, the UV-1900 features high resolution, low stray light, high reproducibility, and an ultra-fast scan function. It also has an easy-to-use interface on a color touch-screen display. The UV-1900 is designed to meet the needs of both high performance and usability.

    Hardware Specifications (UV-1900)

    Item Specification
    Wavelength range 190 to 1,100 nm
    Spectral bandwidth 1 nm (190 to 1,100 nm)
    Wavelength display 0.1nm increments
    Wavelength setting 0.1nm increments (1nm increments when setting scanning range)
    Wavelength accuracy ± 0.05 nm at D2 peak 656.1 nm, ± 0.3 nm for entire range
    Wavelength repeatability ± 0.1 nm
    Wavelength slew rate About 14,500 nm/min
    Wavelength scanning speed 3,000 to 2 nm/min
    Lamp interchange wavelength Automatic interchange linked to wavelength. The interchange wavelength can be set freely in the range of 295 to 364 nm (0.1nm increments).
    Stray light Less than 0.02%  at 220 nm (NaI) Less than 0.01%  at 340 nm (NaNO2) Less than  0.5%  at 198 nm (KCl)
    Photometric system Double beam optics
    Photometric range Absorbance: -4 to 4 Abs Transmittance: 0% to 400%
    Photometric accuracy ± 0.002 Abs at 0.5 Abs ± 0.0025 Abs at 1.0 Abs ± 0.006 Abs at 2.0 Abs (measured using NIST930D/NIST1930 or equivalent.)
    Photometric repeatability Less than ± 0.0001 Abs  at 0.5 Abs Less than ± 0.0001 Abs  at 1 Abs Less than ± 0.0005 Abs  at 2 Abs
    Item Specification
    Baseline stability Less than 0.0003Abs/hr (700 nm, one hour after light source turned ON)
    Baseline flatness Less than ± 0.0006 Abs (1,100 to 190 nm, one hour after light source turned ON)
    Noise level Less than 0.00003 Abs  (700 nm)
    Light source 20-W halogen lamp and deuterium lamp  Built-in light source auto position adjustment
    Monochromator LO-RAY-LIGH grade blazed holographic grating in Czerny-Turner mounting
    Detector Silicon photodiode
    Sample compartment Internal dimensions: W110 × D250 × H115 mm Distance between light beams: 100 mm
    Power requirements AC100,120,220,230,240 V, 50/60 Hz, 140 VA
    Environmental requirements Temperature: 15°C to 35°C Humidity: 30% to 80% (without condensation; 70% max. at 30°C or higher)
    Dimensions W450 × D501 × H244 mm
    Weight 16.6 kg
    Output device USB memory (optional)
    Data files saved in text format or UVPC format. UVPC-format files can be read directly by UVProbe  and LabSolutionsTM UV-Vis.
    PC compatibility UVProbe software (standard) LabSolutions UV-Vis software (optional) External control possible via USB.
    Display 24-bit color touch screen
    Supported languages Japanese, English, Chinese, Spanish (Mexico), Portuguese (Brazil).

    UV-1900 Software Specifications 

    Measurement mode Specification
    Photometric mode Single-wavelength measurement

    1. Photometric modes: T% or Abs
    2. Quantitation using K-factor method
    3. Data table storage and recall functions

    Multiple-wavelength measurement

    1. Photometric modes: T% or ABS
    2. Measurements at up to eight designated      wavelengths (set in 0.1mm increments)
    3. Data calculation at up to four      wavelengths (difference or ratio between      two wavelengths, calculation between      three wavelength, etc.) is possible.
    Spectrum mode
    1. Measurement modes: ABS, T%, E
    2. Number of repeat scans: 1 to 99
    3. Recording system: Selection between    single spectrum and data overlay 4.Data storage and recall 5.Data processing:   Peak/valley detection, arithmetic operations,    differentiation, smoothing, area calculation,    point picking, data reading at    cursor-specified point
    Quantitation mode
    1. Measurement methods:  
      1. wavelength,
      2. wavelength,
      3. wavelength,
      4. and 1st to 4th derivative methods
    2. Quantitation methods:   Automatic concentration calculation using K-factor   Automatic concentration calculation using    single-point calibration curve    Multi-point calibration curve method   (1st to 3rd order regression curves)
    3. Measurement parameters:   Number of standards (2 to 10)   Number of repeat measurements   (1 to 10 times) to obtain a mean value    for quantitation.
    Kinetics mode
    1. Measures absorbance changes as a function of    time and calculates the enzymatic activity values.
    2. Measurement time: 1 to 9,999 sec/min
    3. Measurement methods:
      1. wavelength,  
      2.  wavelength, multi-cell, and rate measurements
    Time scan mode
    1. Measures changes in measured values as a function of time.
    2. Measurement mode: ABS, T%, E
    3. Measurement time: 1 to 9,999 sec/min
    4. Data processing functions (same as spectrum mode)
    Measurement mode Specification
    Biomethod mode DNA/Protein Quantitation

    1. Calculation of DNA/protein concentration and absorbance ratio   DNA concentration = K1 x A1 - K2 x A2   Protein concentration = K3 x A2 - K4 x A1
    2. Factors and measurement wavelengths can be set freely.
    3. Background correction is possible.

    Quantitation of proteins

    1. Quantitation methods: Lowry method, BCA method, Biuret    method, CBB method (Bradford method), UV method
    Maintenance
    1. Baseline correction
    2. Lamp usage time displays and reset
    3. Security settings   Functions can be restricted according to the user.
    4. Instrument validation functions: 
      1. Compatible with 9 JIS items      Wavelength accuracy, wavelength repeatability,       resolution, stray light, photometric accuracy,       photometric repeatability, baseline flatness,       baseline stability, noise level. 
      2. Semi-automatic validation      Validation inspections conducted interactively while inserting and removing inspection jigs.
      3. Fully automatic validation      Automatic validation inspections from measurement to evaluation and printout. 
      4. Setting inspection parameters and pass/fail criteria       Authority to make changes can be protected by password access.
      5. Detailed printout of results 
      6. Bulk printout of results
    Shared functions
    1. Automatic setting of measurement mode after instrument initialization.   It’s possible to specify standby and parameter files in the parameter setting window for each measurement mode.
    2. Selection of the displayed number of decimal places   Absorbance: 3 or 4 decimal places   Transmittance: 1 or 2 decimal places
    3. Number of files that can be saved (built-in memory)   Measurement parameters: 24 files max.   Data: 8 files max.   Tabular data: 8 files max.
    4. The setting of integration time   (for fixed-wavelength measurement)
    5. PC control   Spectrophotometer can be controlled by an external PC.    This function is also used when performing the operation with the UVProbe software provided.   *A USB cable is required.